Abstract
Pathogenic infection is becoming a global health threat to human health. Especially for the treatment of P. aeruginosa remains particularly challenging. Fortunately, it is interestingly found that the LecA and LecB lectins of P. aeruginosa played crucial roles in bacterial adhesion, biofilm formation, virulence, and host cell invasion. Herein, a co-assemble strategy to prepare antibiotic-free antibacterial and antibiofilm agents by using two kinds of perylene-carbohydrate conjugates (PMI-3Gal and PMI-3Fuc) with synergistic targeting for two lectins of P. aeruginosa LecA and LecB was developed. Due to the strong multivalent carbohydrate-lectin interactions both for LecA and LecB lectins, the co-assembly PMI-3Gal@PMI-3Fuc showed selective adhesion effects, inhibition activity of biofilm formation and potent photothermal antibacterial activities for P. aeruginosa and a clinical-isolated P. aeruginosa strain, and showed the acceleration effect for the wound healing in mice. This result opens a supramolecular principle for antibiotic-free antibacterial and antibiofilm effects based on multivalent glycoconjugates.