Affiliation:
1. Teikyo University School of Medicine
Abstract
Abstract
The novel coronavirus SARS-coronavirus 2 (SARS-CoV-2) is the cause of the coronavirus disease 19 (COVID-19), the rapidly spreading pandemic. When SARS-CoV-2 enters the target cell, the spike (S) glycoprotein binds to a cellular receptor angiotensin converting enzyme 2 (ACE2). Effective vaccination has been achieved, utilizing the Spike (S) protein mRNA sequence. Objective: For a full understanding of the effects of Pfizer-BioNTech or Moderna mRNA vaccines, we evaluated the lymphoid responses. We have performed S protein western blots for proteins extracted from axillary lymph nodes, and S protein immunohistochemistry for the axillary lymph node tissues from human autopsies. Results: Our results showed that both vaccinated and control cases (non-vaccinated or negative for anti-S and anti-N antibodies) had positive S protein reactivity in both western blots and immunohistochemistry. This reactivity was present several months after vaccination. One anti-S protein antibody western blots showed a positive correlation with serum anti-S protein amounts. The positivity in non-vaccinated uninfected individuals indicates that either the antibodies against S protein are cross-reacting to other proteins present in human tissues, or due to insidious infections to SARS-CoV-2. Further evaluation is necessary for the reliability of anti-S protein antibodies in SARS-CoV-2 studies.
Publisher
Research Square Platform LLC
Reference18 articles.
1. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury;Kuba K;Nat Med,2005
2. Prevention and Attenuation of Covid-19 with the BNT162b2 and mRNA-1273 Vaccines;Thompson MG;N Engl J Med,2021
3. Baden LR, El Sahly HM, Essink B, Kotloff K, Frey S, Novak R, et al. Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine. New England Journal of Medicine; 2020.
4. Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine;Polack FP;N Engl J Med,2020
5. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses;Turner JS;Nature,2021