Abstract
Background
Amanita is a large genus with extremely rich biodiversity. In its close relationship with humans, more than 90% of the mushroom poisoning events result from the species of this genus. Due to the abundance of the species of Amanita, and the morphological features being relatively conservative, it is difficult to distinguish the species between toxic and edible.
Results
To provide molecular markers and explore the phylogenetic relationship of Amanita genus. We sequenced and analyzed the mitogenomes (Mitochondrial genome) of five Amanita species, and performed a comparative mitogenome analysis combined with the nine published Amanita mitogenomes. In addition, we predicted the introns, repeat sequences, AT-skew, GC-skew, and tRNA (Transfer RNA) for five Amanita species. Four phylogenetic trees of ML (Maximum likelihood) and BI (Bayesian inference) were constructed based on different datasets of 15 PCGs (Protein coding genes) of 55 species, the results showing that this genus was divided into two subgenera, with ectomycorrhizal species and asymbiotic species clustered into one each. Among the 14 Amanita species, the branches and the taxonomic status of all species of Amanita, except A. pseudoporphyria, were identical to the current taxonomic status.
Conclusions
We believe that after a certain amount of mitogenome accumulation, toxic and edible species between the gene rearrangement rules and in its phylogenetic relationship present different distributions. This study provides molecular data for macrofungal studies.