A Human Tooth-On-Chip Model Shows Long-Range Osteoinduction from the Dentine Surface
Author:
Perriman Adam1, Macalester William1, Moreno Rafael1, Shanon Mark1, West N2, Hill Darryl1, Boussahel Asme1ORCID
Affiliation:
1. University of Bristol 2. Univ Bristol, Sch Dent, Div Restorat Dent, Lower Maudlin St, Bristol BS1 2LY, Avon, England
Abstract
Abstract
Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral defects. However, the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering, exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems, which will impact on cell fate and subsequent treatment efficacy. Herein, we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells (hMSCs) encapsulated in a microporous hydrogel bioink. We demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs, and that both dentine and b-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface, which is distal to the gel-substrate interface. Our findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provides a framework for the rapid development of 3D dentine-bone interface models.
Publisher
Research Square Platform LLC
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