Microtomographic analysis of surgically-created bone defects in rats: manual versus predefined region-of-interest definition

Author:

Pitorro Tássio Edno Atanásio1,Irie Milena Suemi1,Bites Clara de Oliveira Barbosa1,Spin-Neto Rubens2,Soares Priscilla Barbosa Ferreira1

Affiliation:

1. Federal University of Uberlândia

2. Aarhus University

Abstract

Abstract Micro-computed tomography (micro CT) is a high-resolution radiographic modality that has been widely used in bone-tissue research. This study assessed whether there is an agreement between the manual and predefined region-of-interest (ROI) definition methods during the three-dimensional analysis of bone defects by micro-CT. Bone defects of 2.3 mm in diameter were created in the left tibiae of Wistar rats. After 14 days, the tibiae were collected and scanned. During the analysis by CtAnalyser® software, the ROI was defined in two different ways for each sample: manually (manualROI) and based on a predefined circular shape (circularROI). Data corresponding to bone volume fraction (BV/TV) and trabecular number (Tb.N) were analyzed. No difference was found between the groups for both BV/TV (p = 0.51) and Tb.N (p = 0.38), with an excellent correlation for BV/TV (r = 0.9). A non-significant correlation was observed for Tb.N (p = 0.1). The Bland–Altman analysis showed a low systematic error for BV/TV (1.2%) and Tb.N (0.2/mm). The manualROI was more time-consuming (512.8 ± 60.5 seconds) than the circularROI method (95.4 ± 9.4 seconds). Both methods provided similar results, therefore, predefined formats for ROI definition of regular lesions are indicated, considering their use is less time-consuming.

Publisher

Research Square Platform LLC

Reference31 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3