Molecular detection of Coxiella burnetii infection in patients with infectious endocarditis culture negative after cardiovascular surgery in Vietnam

Abstract

Abstract Objective: To detect Coxiella burnetii in endocarditis patients cultured negative for bacteria in a short-term study from January 2022 to February 2023 at Cardiovascular Surgery Unit, Bach Mai Hospital. Materials and methods: 312 Patients with endocarditis diagnosed and operated at the Department of Cardiovascular Surgery - Bach Mai Hospital were included in the study after obtaining the consent of each study subject and the consent of the participants. hospital ethics committee. After surgery, the patient's blood sample was cultured to identify bacteria by an automatic system at the Department of Microbiology of Bach Mai hospital, 52 case was the result is negative, it will continue to be PCR analyzed with Coxiella burnetii primer pair at the Department of Microbiology, University of Natural Sciences, Vietnam National University, Hanoi. Results: In a short study with 52 blood samples from endocarditis patients who were found to be negative for bacteria, 13samples were positive for Coxiella by PCR. Among 9 patients, there were 6 patients with fever lasting 14 days after surgery, 6patients with emaciation, pneumonia, 2patient with multi-organ failure, emaciation.