Association of Angiotensin-I-Converting Enzyme (ACE) Insertion/ Deletion Gene Polymorphism with Preeclampsia Susceptibility in Egyptian women

Abstract

Abstract The current study was designed to investigate the association of angiotensin-I-converting enzyme (I / D) gene polymorphisms with the susceptibility and clinical pattern of preeclampsia among Egyptian cases from the Nile Delta region. Oxidative stress biomarkers, including Serum levels of malondialdehyde, glutathione-s-transferase, superoxide dismutase, nitric oxide, glutathione peroxidase, endothelin-1, and interleukin-6 as a pro-inflammatory biomarker, were assessed by Elisa. In this study, genotyping, and allelic frequencies of the ACE I/D (rs4646994) variant with preeclampsia susceptibility was about 38.2%. In addition, the results revealed that the ACE/ID (rs4646994) genotype distribution was associated with preeclampsia in the studied population from the Nile Delta region of Egypt. Frequencies of the D allele and DD genotype were significantly increased, while frequencies of the I allele and ID genotype were significantly decreased in the preeclampsia patients when compared with the control subjects (P = 0.014, OR = 1.94, 95% CI = 1.254–3.233 for DD) and (P = 0.016, OR = 1.4, 95% CI = 1.061-2.10 for D). Our findings suggest the association between rs4646994 and susceptibility to preeclampsia in Egyptian cases from the Nile Delta region. Examining the impact of ACE gene variants on preeclampsia may be a valuable method for locating and treating women at risk. Moreover, the angiotensin-I-converting enzyme DD genotype and D allele were associated with preeclampsia as risk factors for Egyptian women. There was a significant association between the serum levels of oxidative stress biomarkers and pro-inflammatory biomarker of preeclampsia compared to the healthy group, with P < 0.05.