Method Development for Simultaneous Estimation of Acetaminophen and Its Related Impurities by Liquid Chromatography-mass Spectrometry

Abstract

Abstract Development and Analysis of drugs involve the most stimulating work in the medicinal field in that way, in that way, there emergence of new drug in day to day. Acetaminophen is a common Over the counter drug used by worldwide as the use of this drug increased globally requirement for detecting its impurities is mandatory and its determination of related substance were evaluated by using Liquid Chromatography Mass spectrometry, this method has emerged as a capable and multipurpose tool for analysing various types of drug product and its impurities. It has the capability of detecting small molecule to high molecule with high sensitivity. The optimized method was developed for estimation of related substance of acetaminophen using a gradient program mobile phase of formic acid and acetonitrile with 0.6 ml/min flow at 210 and 254 nm wavelength with optimized mass condition. In this paper, briefly discussed about method development for determination of related substance of acetaminophen along with its active pharmaceutical ingredient in a finished marketed product.