Affiliation:
1. Henan Agricultural University
2. Weinan Animal Health Workstation
3. Department of Agriculture and Rural Affairs of Urumqi
4. China Agricultural University
Abstract
Abstract
To monitor benzoic acid (BA) residues in liquid food samples, a monoclonal antibody (mAb)-based lateral flow immunoassay (LFA) was developed in this study. First, 2-aminobenzoic acid (2-AA), 3-aminobenzoic acid (3-AA), and 4-aminobenzoic acid (4-AA) were conjugated to BSA and used as immunogens. After cell fusion, mAb 6D8 from 4-AA-BSA performed best with an IC50 value of 0.21 mg/L using 3-AA-OVA as heterogeneous antigen, which represented a 3.4-fold improvement compared with the homogeneous antigen 4-AA-BSA. Subsequently, eight kinds of CGNPs with sizes varying from 20.94 nm to 90.00 nm were synthesized for screening the suitable size to develop a sensitive LFA. Finally, a sensitive LFA based on colloidal gold (23.27nm) nanoparticles was developed for screening BA with a cut-off value of 4 mg/L, which could meet the requirement of BA detection in milk, Fanta, Sprite, Coca-Cola, and Stmart samples.
Publisher
Research Square Platform LLC