Genetic Polymorphisms of Genes Coding for Cytokines, IL6 -174G>C (rs1800795) and IL10 -1082A>G (rs1800896), in Cervical Insufficiency

Author:

Karakus Nevin1,Gulucu Selim1,Onal Mesut2,Yildirim Sumeyye1

Affiliation:

1. Tokat Gaziosmanpasa University

2. Ondokuz Mayis University

Abstract

Abstract

A recurring, passive, and painless dilatation of the cervix in the second trimester is known as cervical insufficiency. Although the exact cause is unknown, subclinical intra-amniotic infection may be linked to it. The purpose of this research is to see if there is a link between interleukin 6 (IL6) -174G > C (rs1800795) and interleukin 10 (IL10) -1082A > G (rs1800896) polymorphisms and cervical insufficiency. DNA samples from 93 patients with cervical insufficiency and 103 healthy controls were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. Statistical analysis was performed using the chi-square test. Any statistically significant difference wasn’t found between cervical insufficiency patients and healthy controls in terms of allele and genotype distributions of IL6 -174G > C and IL10 -1082A > G polymorphisms (p > 0.05). However according to composite genotyping analysis, CC/AA (IL6 -174G > C / IL10 -1082A > G) composite genotype revealed a significant difference between patients and controls (p = 0.049). Number of abortion and CC/AA (IL6 -174G > C / IL10 -1082A > G) composite genotype was also associated with each other (p = 0.006). Although we did not find an association between IL6 -174G > C (rs1800795) and IL10 -1082A > G (rs1800896) polymorphisms and cervical insufficiency individually, it seems that these polymorphisms together may predispose to the disease and its severity.

Publisher

Research Square Platform LLC

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