Evaluation of colonic anastomosis healing using hybrid nanosheets containing Molybdenum disulfide (MOS2) scaffold of human placental amniotic membrane and Polycaprolactone (PCL) In rat animal model

Abstract

Abstract Purpose Anastomosis is a standard technique following different conditions, such as obstruction, tumor, and trauma. Obstruction, adhesion, or anastomosis leakage can be some of its complications. To improve healing and prevent postoperative complications, we design a hybrid scaffold containing acellular human Amniotic membranes and Polycaprolactone-Molybdenum disulfide nanosheets for colon anastomosis. Methods The animal model of colocolonic anastomosis was performed on two groups of rats; control and scaffold. The hybrid scaffold was warped around the anastomosis site in the scaffold group. Samples from the anastomosis site were resected on the third and seventh postoperative days for histopathological and molecular assessment. Results Histopathologic score and burst pressure had shown significant improvement in the scaffold group. No mortality and anastomosis leakage was reported in the scaffold group. In addition, inflammatory markers were significantly decreased, while anti-inflammatory cytokines were increased in the scaffold group. Conclusion The result indicates that our hybrid scaffold is a proper choice for colorectal anastomosis repair by declining postoperative complications and accelerating healing.