Preliminary study of LACTB transcript 1 inhibiting gastric cancer cell migration and invasion by regulating autophagy

Abstract

Abstract Purpose: To analyze the expression level of β-lactamase gene (LACTB) in gastric cancer cells and the main types of transcripts expressed, and then explore the effect and mechanism of LACTB transcripts on the biological function of gastric cancer cells. Methods: The expression level of LACTB and the main biological processes involved were analyzed by bioinformatics analysis and RT-qPCR. The expression level of LACTB transcript 1 was overexpressed or knocked down in AGS and HGC-27 cells, and the effect and mechanism of LACTB on gastric cancer cell migration and invasion were verified by in vitro cell experiments and western blotting experiments. Results: 1.The UALCAN analysis website showed that LACTB mRNA was highly expressed in gastric cancer tissue, and the expression level of LACTB transcript 1 in peripheral blood of gastric cancer patients was consistent with the bioinformatics analysis. LinkedOmics and DAVID enrichment analysis found that LACTB co-expressed genes were mainly involved in biological processes such as immune response, inflammatory response, and proteolysis through lysosomes and immune synapses. 2.Knocking down the expression level of LACTB transcript 1 can inhibit the migration and invasion ability of gastric cancer cells, and at the same time can activate autophagy and inhibit EMT. However, overexpression of LACTB transcript 1 resulted in the opposite. Conclusions: LACTB transcript 1 is an inhibitor that regulates autophagy to inhibit the migration and invasion of gastric cancer cells, and is highly expressed in peripheral blood, which has the potential as a biomarker of gastric cancer.