Combining different ion-selective channelrhodopsins to control water flux by light

Author:

Lin Fei1,Tang Ruijing2,Zhang Chong1,Scholz Nicole3,Nagel Georg1,Gao Shiqiang1

Affiliation:

1. Julius-Maximilians-University of Würzburg

2. Mengchao Hepatobiliary Hospital of Fujian Meidical University

3. Leipzig University

Abstract

Abstract Water transport through water channels, aquaporins (AQPs), is vital for many physiological processes including epithelial fluid secretion, cell migration and adipocyte metabolism. Consequently, modulation of water transport poses significant therapeutic potential in edema, cancer, obesity, brain injury, glaucoma etc. Water flux through AQPs is driven by the osmotic gradient that results from concentration differences of small molecules or ions. Here, we developed a novel optogenetic toolkit that combines the light-gated anion channel GtACR1 either with the light-gated K+ channel KCR1 or the new NCR1 with high Na+ permeability, to manipulate water transport in Xenopus oocytes non-invasively. Water efflux through AQP was achieved by inducing K+ and Cl efflux through light-gated HcKCR1 and GtACR1, respectively. Contrarily, when GtACR1 was co-expressed with NCR1, a final inward movement of Na+ and Cl was triggered, and an osmotic gradient was formed to drive water influx through AQP1. In sum, we demonstrated a novel strategy to manipulate water movement into and out of Xenopus oocytes non-invasively through light. These findings provide a new avenue to interfere with water homeostasis as a means to study related biological phenomena across cell types and organisms.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3