Dose-dependent effect of canine lyophilized platelet on an in vitro hemodilution model

Abstract

Abstract The transfusion of stored platelet has emerged as an efficient method for treating dogs with thrombocytopenia. However, the availability of fresh platelet is limited in veterinary medicine due to demanding storage conditions. Lyophilized platelets have long shelf lives and can be easily stored, increasing their accessibility for thrombocytopenic dogs. Due to the lack of information on the appropriate dose of lyophilized canine platelet, the platelet transfusion dosage, approximately 1×1011/m2 in veterinary practice was extrapolated from human studies. This study was to evaluate the dose effect of lyophilized canine platelet on blood coagulability and to provide a basis for the clinical application. Three different concentrations of lyophilized canine platelets were added to in vitro hemodilution blood model, increasing the platelet count by 25, 50, and 100 ×106/ml and coagulation profiles were analyzed. The coagulability profiles showed tendencies to improve as the transfusion dose increased. These results revealed the possibility that the higher dose of canine lyophilized platelets than the standard dose, currently used in veterinary clinics, may show better clinical outcomes.