Abstract
Purpose:
Recent research suggests that combining nanoselenium supplements with exercise enhances immune function. This study investigates Influence of Nanoselenium Supplementation and Interval Training on Hepatic MMP-13 and TGF-β Gene Expression in Dexamethasone-treated Rats.
Methods:
Forty eight-week-old rats were randomly assigned to five groups:1-Control group (healthy rats). 2-Dexamethasone-induced control group. 3-Dexamethasone + nanoselenium supplement group. 4-Dexamethasone + interval exercise group. 5-Dexamethasone + nanoselenium supplement + interval exercise group.Interval training involved running repetitions on a treadmill with increasing intensity and duration over four weeks. Nanoselenium supplements were orally administered every other day.
Results:
Finding demonstrated a significant increase in MMP13 expression in Dexa, NaSe, Trng and Trng+NaSe groups than Control group (p<0.05). NaSe, Trng and Trng+NaSe groups exhibited significantly lower MMP13 expression compared to the Dexa group (p<0.0001). The combination of training with nanoselenium resulted in a significant reduction in MMP13 expression compared to training alone (p<0.05).The Dexa group exhibited a significant increase in TGF-β levels, with an expression more than twofold higher than the control group (p < 0.01). TGF-β expression was significantly lower in the NaSe (p < 0.0001), Trng (p < 0.05), and Trng+NaSe (p < 0.0001) groups compared to the Dexa group. In addition, TGF-β expression in the Trng+NaSe group was significantly lower than that in the Trng group (p < 0.05).
Conclusion:
Intermittent exercise and nanoselenium supplementation enhance the body's antioxidant system, leading to improved immune function and the mitigation of the negative effects of dexamethasone on MMP-13 and TGF-β gene expression.