Improving biomass and dendrobine-type totalalkaloid (DTTAs) production of Dendrobium nobile through combining Temporary Immersion Bioreactor system (TIBS) with Endophyte MD33 elicitation

Abstract

Abstract Dendrobium nobile Lindl. (D.nobile) is a perennial herb of the orchid family and is commonly used in traditional Chinese medicine. Due to the scarcity of D.nobile resources, the market is facing a shortage. To meet market demand, enhance cultivation efficiency, and reduce costs, the temporary immersion bioreactor (TIBS) has been employed for cultivation. The optimal cultivation conditions for D.nobile tissue culture seedlings have been determined through experimental research. These conditions include a liquid medium consisting of 1/2 MS, 30 g/L sucrose, 80 g/L potato, 0.5 mg/L NAA, 60 g/L banana, and a pH of 6.0. The optimal inoculation volume of TIBS per canister was 220 D.nobile seedlings, and the optimal submergence frequency of TIBS was set at 5 min/8 h, and the optimal submergence time was set at 6 min/6 h. In addition, it was observed that its endophytic fungus MD33, had a significant impact on the dendrobine-type total alkaloid (DTTAs) content and plant growth. This study demonstrates that utilizing TIBS for cultivation and co-cultivating with endophytic fungus significantly increased the biomass and bioactivity of D.nobile tissue culture seedlings.