Ultrafast Bioanalytical Assay for Vilazodone Quantification in Human Plasma Using Vilazodone D8 Internal Standard by Uplc- Ms/ms

Author:

Agrawal Nikhil1,Mittal Amit1

Affiliation:

1. Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar-Delhi G.T. Road (NH-44), Phagwara (Punjab) 144411

Abstract

Abstract A selective and highly sensitive quantitative method has been established for assessment of pharmacokinetic parameters in human plasma using vilazodone D8 as a labelled internal standard. Liquid- Liquid extraction technique (LLE) was applied for plasma sample extraction. Mass detection was performed in positive electro spray ionization method. Quantitation was achieved by monitoring sum multiple transitions of m/z 442.022 → 155.000 + 197.000 for vilazodone and 450.093 → 157.000 + 205.000 for vilazodone D8. Chromatographic separation was performed on reverse phase Betabasic C8, 100*4.6mm, 5µ column with 0.700mL/min flow rate. Mobile phase consists of acetonitrile and 0.1% formic acid in water (60:40%v/v) was pumped through isocratic mode. The linearity of the method was validated from range 0.300ng/mL to 300.000ng/mL. Precision and accuracy batches were found to be consistent, reproducible and acceptable within the defined limits across the validation. No matrix effect was observed within the validated range and extraction efficiency or recovery was found to be consistent and reproducible at all concentration levels (low, middle and high). The stock solutions, working solutions, plasma samples and processed samples were found to be stable under all defined conditions. In this validated method, selective linearity range were used to cover quantitative analysis for various strength formulations. This work was typically aimed to develop a method with shorter analysis time and simple extraction procedure for reliable measurement of clinical samples. The validated method can be useful in determining plasma concentration of vilazodone for therapeutic drug monitoring and in high throughput clinical bio-studies.

Publisher

Research Square Platform LLC

Reference35 articles.

1. Vilazodone for the treatment of depression: an update;Wang SM;Chonnam medical journal,2016

2. VIIBRYD (vilazodone hydrochloride) Initial U.S. Approval: 2011 Available from https://www.accessdata.fda.gov/drugsatfda_docs/label/2015/022567s012lbl.pdf.

3. Vilazodone: clinical basis for the US Food and Drug Administration's approval of a new antidepressant;Laughren TP;The Journal of clinical psychiatry,2011

4. Quantification of selected antidepressants and antipsychotics in clinical samples using chromatographic methods combined with mass spectrometry: A review (2006–2015);Sistik P,2016

5. Frampton, J. E. (2011). Vilazodone. CNS drugs, 25(7), 615–627.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3