Affiliation:
1. Sichuan Agricultural University
2. Zhengzhou University of Light Industry
3. Chinese Academy of Agricultural Sciences
Abstract
Abstract
The fast-growing rhizobia-like strains S101T and S153, isolated from nodules of Glycine max in Sichuan, P.R. China, were characterized by using a polyphasic taxonomy approach. Growth was observed at 20–40°C (optimum, 28°C), pH 4.0–10.0 (optimum, pH 7) and with up to≤2.0% (w/v) NaCl (optimum, 0.01%) on Yeast Mannitol Agar (YMA) plates. 16S rRNA genes of strain S101T and Ciceribacter daejeonense L61T were 98.4% similar. The major cellular fatty acids of strain S101T were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The DNA G+C contents of S101T and S153 were 61.1 mol% and 61.3 mol%, respectively. The digital DNA–DNA hybridization (dDDH) relatedness and average nucleotide identity (ANI) values between S101T and the most closely related type strain C. daejeonense L61T were 46.2% and 91.4–92.2%, respectively. Therefore, we propose that the strains S101T and S153 represent a novel species with the name Ciceribacter sichuanense sp. nov. and strain S101T as the type strain (CGMCC 1.61309T= JCM 35649T).
Publisher
Research Square Platform LLC
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