Comparative study of a nano-bacterial rat kidney stone model and the traditional ethylene glycol rat kidney stone model

Abstract

Abstract Objective To Compare a nanobacterial (NB) rat kidney stone model and the traditional ethylene glycol (EG) rat kidney stone model to assess its significance. Methods Ninety Wistar male rats were randomly divided into three groups. After the first week of modeling, three rats of every groups were randomly selected to measure the biochemical blood and urine markers. After the sacrifice, the renal tissues were observed to assess the pathological changes. The expression levels of CaSR and Claudin-14 protein were detected by kinds of technology. Results The biochemical metabolic indices of rats in NB group began to increase at the third week and decreased to normal at the 9th week. In the EG group they began to increase at the second week and did not return to normal later. At the 7th week, the creatinine levels of rats in the EG group were higher than in the NB group, and the difference was statistically significant. The formation rates of kidney stones in the NB group and EG group were 52.4 and 66.7%, respectively, but the difference of the two rates was not statistically significant. The protein expression CaSR and Claudin-14 in the EG group began to strengthen at 3rd week, and at 4th week in the NB group. CaSR was continuously expressed in the NC group, but Claudin-14 was not expressed. Conclusion The formation of stones in the NB group began slightly later. CaSR and Claudin-14 proteins play a role in the formation of kidney stones.