Affiliation:
1. Tarbiat Modares University
2. Baqiyatallah University of Medical Sciences
Abstract
Abstract
We present an advanced electrochemical immunosensor designed for precise detection of the Vascular Endothelial Growth Factor (VEGF). The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employing thermal annealing and a de-alloying process, the silver is eliminated from the electrode, resulting in a reproducible porous gold substrate. Utilizing a well-defined protocol, we immobilize the heavy-chain (VHH) antibody against VEGF on the gold substrate, facilitating VEGF detection through various electrochemical methods. Remarkably, this immunosensor achieves an outstanding performance, featuring an impressive detection limit of 0.1pg/ml and an extensive linear range from 0.1pg/ml to 0.1µg/ml. This emphasizes its exceptional ability to precisely measure biomarkers across a wide concentration spectrum. The robust fabrication methodology employed in this research underscores its potential for widespread application, offering enhanced precision, reproducibility, and remarkable detection capabilities for the developed immunosensor.
Publisher
Research Square Platform LLC