Abstract
MIKC-type genes play crucial roles in the growth and development stages of plants. Identifying MIKC-type genes in wheat and exploring their expression patterns under low-temperature conditions lays a foundation for subsequent research on gene function. Bioinformatics websites and software were utilized to identify MIKC-type genes in the wheat transcriptome under low-temperature conditions, and the expression changes of these genes were verified using RT-qPCR methods. A total of 90 MIKC-type genes were identified, which could be classified into two major categories and nine subfamilies, distributed across 21 chromosomes. There were numerous inter-chromosomal duplications of MIKC-type genes in wheat. Transcriptome analysis revealed that under low-temperature conditions, the expression of 14 MIKC-type genes was altered, with seven genes significantly upregulated and three genes significantly downregulated. The validation results for TaMIKC30 and TaMIKC68 were consistent with the transcriptomic data, and the results also indicated that the expression of these two genes differs slightly between different tissues. These findings suggest that MIKC-type genes may be involved in the response of wheat to low-temperature stress.