Evaluation of Insecticidal activity and Genetic Expressions of some Essential Oil and Methomyl Lannate 90% against Spodoptera frugiperda [J.E. Smith]; (Lepidoptera: Noctuidae)

Abstract

Abstract Fall Armyworm (FAW) Spodoptera frugiperda [J.E. Smith] (Lepidoptera: Noctuidae) is regarded as a major pest of various economic crops, their caterpillars are a highly destructive and have a wide host range. This study's goal was to assess the insecticide potency of essential oils [rosemary (Rosmarinus officinalis L.), lemongrass (Cymbopogon citratus) and Cinnamon (Cinnamomum zeylanicum)] and Methomyl Lannate 90% commerial Insecticide to control FAW and their effected on Expression of caspase-8 and Inhibitor of Apoptosis Protein (IAP) genes and Expression of Acetylcholinesterase (AChE) gene in FAW. The insecticidal activity against 2nd larval instar was evaluated with five concentrations (2.5%, 2%, 1.0%, 0.5% and 0.25%) for essential oil and four concentrations 0.4%, 0.2%,0.15% and 0.05% for Methomyl Lannate 90%. The findings indicated that raising both essential oil concentrations and Methomyl Lannate 90%, resulted in increased the larval mortality at high concentration. Lemongrass and Cinnamon oil produced about to the same estimated LC50, whereas Methomyl Lannate 90% was (0.3%). Cymbopogon citratus (1.68%) had higher Caspase-8 gene expression levels compared to the control, but at a lower level than Rosmarinus officinalis L. (2.59%) and Cinnamomum zeylanicum (1.67%). The expression levels of Ache gene in the treated by low concentration of Methomyl Lannate were increased (by 313%) significant compared with the control but without significant differences. FAW death as a result of treatment with the tested oils having an effect on the genes that the pest uses to express critical processes. As a result, using the tested essential oils as a substitute for conventional management of FAW is a brilliance option.