Epidemiological survey and Genotype diversity of Mycoplasma pneumoniae in Guangxi China swine herds based on MLST

Abstract

Abstract Background Mycoplasma hyopmneumoniae (Mhp) is a swine pathogen bacterium, causing significant economic losses worldwide. In this study, total of 1362 samples of swine lung from 14 cities in Guangxi were collected randomly. And TaqMan Real-time PCR Detection Method was applied in the detection of Mhp. The strongly positive samples were used in the multilocus sequence typing (MLST). The purpose of this study is to investigate the prevalence of Mhp in Guangxi and to reveal the genotypes and distribution of Mhp in swine herds in Guangxi based on MLST. Results Out of 1362 samples, 655 samples were detected mhp positive. Among them 61 samples were successfully amplified three housekeeping genes (adk, rpoB and tpiA) simultaneously using MLST method. What is more, this study recorded 27 sequence types from 14 cities in Guangxi Province, including 24 new STs. From genetic evolution tree analysis, it can be revealed that the prevalent strains of Mhp in Guangxi, China are types II and V. All in all, the Mhp popular in Guangxi China have a high genetic diversity. Conclusions This is one of the most comprehensive study available for reporting the prevalence and molecular typing of Mhp in Guangxi China which will enrich the local database of Mhp prevalence in China and provide a theoretical basis for the prevention and control of Mhp in the Guangxi region of China.