The differentiation of hematopoietic stem cells was disturbed in advanced tumor-bearing mice

Abstract

Abstract Background Hematopoiesis have been proved that it could be changed in the patients with advanced cancer. In this study, we investigated the changes of HSCs differentiation in advanced tumor-bearing mice. Methods The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of B16-F10 mouse melanoma cells into the right back of male wild-type C57BL/6 mice. Hematopoietic stem cells and multi-lineage differentiation were evaluated using blood routine, HE-staining, flow cytometry assay and HSCs culture technology. Results The mice model exhibited hematopoietic suppression, marked by a severe anemia, and the multi-lineage differentiation of hematopoietic stem cells was disturbed. Especially, the differentiation of megakaryocyte and erythrocyte were blocked, while myeloid cell and lymphoid cell differentiation was encouraged in advanced tumor-bearing mice. Conclusion In this study we showed that the differentiation of hematopoietic stem cells was disturbed in advanced tumor-bearing mice, which provided new knowledge about cancer cachexia related hematopoietic abnormality.