Abstract
Objective:
This study investigates genotypic variations in GST-M, GST-T, and TP53;rs1042522 among children with obesity.
Methods:
Blood samples from 60 patients with childhood obesity were analyzed. Deletions in GST-M and GST-T were identified using quantitative PCR with melting curve analysis, while TP53;rs1042522 was genotyped via sequence analysis. Deviation from Hardy-Weinberg proportion was examined, and associations with clinical and demographic variables were assessed.
Results:
We observed deviations in the genotypes of GST-M and GST-T, while TP53;rs1042522 remained aligned. Higher cholesterol, LDL, and GGT levels were found in individuals with null GST-M genotypes. Notably, individuals with Wt/null GST-T genotypes had remarkably higher waist circumference and levels of albumin. The wild-type GST-T genotype correlated with reduced BMI and creatinine levels. Individuals with TP53;rs1042522 mutations showed decreased LDL and cholesterol levels but increased ALT levels.
Conclusion:
The research highlighted the notable influence of genetic variations in GST-M and GST-T on obesity in children, while the TP53 polymorphism, rs1042522, did not show a significant impact.