Abstract
Arsenic exposure has been known to be associated with the male reproduction injury. Exploring the antidote of arsenic and ascertaining proper dose of antidote are important for detoxifying the male reproductive toxicity of arsenic. Selenium, which is essential for the male reproduction and spermatogenesis, can alleviate the toxicity of many environmental toxins, such as metals, and F. Selenium relives arsenic-induced reductions in spermatogenesis index and testicular function marker enzymes via promoting the antioxidative ability of rats. Our previous study has found that arsenic can induce reproductive toxicity by affecting the level of H3K14ac in the testis, so we further investigate whether selenium can antagonize arsenic-induced male reproductive toxicity through the H3K14ac pathway, and ascertain the appropriate dose of selenium. The results show that selenium intervention reduce the accumulation of arsenic in rat testis probably attributing to promote the excretion of arsenic from rat, then improve the testis injury induced by arsenic. Selenium intervention enhances sperm quality, testosterone level and expression of steroidogenic genes by regulating H3K14ac level and expression of its associated enzymes (KAT2A, P300, BAZ2A, HDAC3 and HDAC6), thus alleviates the male reproductive toxicity of arsenic and the proper dose of Se for mitigating arsenic male reproductive toxicity is 1mg/kg.