Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities

Abstract

AbstractCollagen-based hydrogels as scaffolds are one of the most widespread biological materials used in biomedicine and drug carriers. The aim of the current study was to develop a vitamin E (VE) loaded hydrogel films based on fish scale collagen (FSC) and carboxymethyl cellulose crosslinked with triethanolamine (TEA) to evaluate their Vitamin E release efficiency, antibacterial and antioxidant properties. The effect of different collagen content on film properties was investigated. The physical and morphological properties of the extracted FSC and the films were determined different analytical technics. The in-vitro properties of the films, the loading and releasing of VE showed that the highest amount of VE loading (98.1%) and release (92.3%) was achieved for the 14.1 wt% collagen film. The antibacterial assay of the prepared film againstE. coliandS. aureusindicated a 2log reduction after 24 h, corresponding to 99% percent reduction. Based on DPPH radical scavenging activity, FSC/CMC/TEA/VE film showed scavenging activity when compared with standard Vitamin C and FSC/CMC/TEA. Moreover, according to the results of MTT test, the film has no toxic effect. Based on these results, the prepared hydrogel film seems to be a promising option in the field of drug delivery.