The improvement of kinsenoside in wild-imitated cultivation Anoectochilus roxburghii associated with endophytic community

Abstract

AbstractBackground Kinsenoside, a predominant pharmacologically active component in Anoectochilus roxburghii, exhibits great liver protective effect for treating non-alcoholic steatohepatitis, autoimmune hepatitis, alcoholic liver injury, and liver fibrosis, etc. Previous research found that the content of kinsenoside in wild-imitated cultivation A. roxburghii (WC) was similar to that of wild-tending cultivation plants (WP). This manuscript disentangled the relationship between kinsenoside and microbiome of A. roxburghii under wild-imitated environment. Results Our research found that endophytic bacteria’s diversity and network complexity decreased from WP to WC and tissue-cultured plants (TC). After 3 months of growth in the wild-imitated environment, the microbial composition of TC was significantly differentiated and converged toward WP. Correlation analyses showed that the diversity of bacterial community has a significant positive relationship with the content of kinsenoside, which is the main functional active ingredients inA. roxburghii, andBCPis the key taxa affecting kinsenoside. In addition, acetylacetyl-CoA reductase, a key enzyme for the synthesis of kinsenoside, was significantly enriched in WC and WP. Conclusion we proposed that the medicinal quality ofA. roxburghiiunder the wild cultivation imitated method is comparable to wild tending cultivation ones with high kinsenoside content which is likely attributed to the activity ofBCP andacetylacetyl-CoA. This study provides a basis for the improvement and sustainable utilization ofA. roxburghiiresources.