MicroRNA-595: A novel circulating biomarker for prediction the status of cerebral collateral circulation in acute ischemic stroke

Abstract

Abstract Objective This study aimed to investigate the correlation between the circulating miR-595 level and the status of cerebral collateral circulation (CCC), and to investigate whether miR-595 could serve as a potential biomarker for the status of CCC in acute ischemic stroke patients. Material and methods This prospective study consecutively enrolled acute ischemic stroke patients and the grouping was as followed according to ASPECTS system: patients with grades > 8 (benign CCC group) and patients with grades ≤ 8 (poor CCC group). Plasma miR-595 was measured by RT-PCR. Spearman rank correlation analysis and logistic regression analysis were used to examine associations between circulating miR-595 level and CCC. Receiver operating characteristic (ROC) curves were performed to determine circulating miR-595 cutoff level scores. Results 106 patients were enrolled (51 with benign CCC and 55 with poor CCC). Plasma miR-595 was significantly higher in the benign CCC group compared with poor CCC group (P < 0.05). miR-595 was positively correlated with ASPECTS system (r = 0.567, P < 0.001), and negatively correlated with NIHSS on admission (r =-0.301, P = 0.002). Patients with higher circulating miR-595 level had a higher risk of benign CCC (OR = 1.122, 95% CI, 1.034,1.218) after adjusting traditional risk factors. ROC curves revealed the areas under the curve for circulating miR-595 were 0.8431 (95% CI 0.533–0.793). Conclusions This study indicated that plasma miR-595 level might be a novel potential prognostic biomarker for benign CCC after acute ischemic stroke onset, which might assist in the administration of thrombolysis and accurately preclinical assessment.