Isolation of Pseudomonas aeruginosa from Food and Determination of Their Antibiotic Resistance

Abstract

Abstract Despite the fact that Pseudomonas aeruginosa is as a prominent opportunist pathogen, it is a recondite microorganism in food safety. Due to its high adaptation capability, rapid reproduction and minimal growth necessities, P. aeruginosa is a common bacterium in the food chain. The resistance of P. aeruginosa to various antibiotics in clinical isolates has been extensively investigated and in-depth characterized. Conversely, information on food-borne isolates of this genus and species is insufficient. Therefore, in this study, 208 P. aeruginosa strains were isolated from 144 food samples collected from different provinces, and 10 isolates (from milk samples) with P. aeruginosa-specific characteristics were identified according to their 16S rRNA region. Antibiotic resistances of the identified isolates were determined against azithromycin (macrolides), erythromycin (macrolides), cephoperazon (cephalosporin), ceftazidime (cephalosporin), meropenem (carbapenem), imipenem (carbapenem), amikacin (aminoglycoside), teminoglycosylcin (teminoglycoside), teminoglycosylcin (pinocycycin) antibiotics. In this study, P. aeruginosa isolates were isolated from milk and the results showed that the prevalence of antibiotic resistance was low. It was found that all of the isolates showed 100% sensitivity to imipenem, piperacilin and vancomycin, 90% sensitivity to cephaperazon and meropenem, and 80% sensitivity to amikacin, ceftazidime and gentamycin. The same isolates were found to be 50% resistant to azithromycin, 90% resistant to tetracycline, and 100% resistant to erythromycin, nalidixic acid, ampicillin and chloramphenicol.