Abstract
Background: Epidermal inflammation, often triggered by environmental exposure or pathological conditions, is intricately associated with tissue damage and various skin diseases.
Methods:This study employed network pharmacology and cell experiments to validate the anti-inflammatory mechanism of Paris polyphylla.
Results: Paris polyphylla targets crucial inflammation-related genes such as IL6, IL-1B, TNF, and CXCL8 within its protein-protein interaction (PPI) network. Pathway analysis revealed significant enrichment not only in inflammatory factor processes but also in toll-like receptor, MAPK, and NF-κB pathways. Key nodes in the PPI network include TLR4, MAPK3, and MYD88. Molecular docking demonstrated robust binding of Polyphyllin H, a component of Paris polyphylla, with MAPK3 and MYD88, suggesting its potential in modulating skin inflammation. Paris polyphylla exhibited the ability to attenuate the upregulation of IL-1β, IL-6, IL-8, and TNF-α in both UVA-damaged and LPS-bacteria stimulated HaCat models.
Conclusions: Thus, Paris polyphylla may regulate the TLR4 pathway through MYD88 and MAPK3, alleviating epidermal inflammation induced by MAPK pathway and NFκB activation. These findings underscore the promise of Paris polyphylla in ameliorating skin damage and diseases, underscoring its significance in dermatology and cosmetics.