In vitro and in silico analysis of the effect of fluconazole, an antifungal drug, on DNA

Author:

Zarf Sumeyye1,Arman Ibrahim1

Affiliation:

1. Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Zonguldak Bulent Ecevit University, Zonguldak, Turkey

Abstract

Fluconazole is an important antifungal drug used worldwide for the treatment of peritonitis and cryptococcal meningitis, urinary tract infections, esophageal tract infections and vaginal candidiasis. In this research, pUC19 plasmid DNA was treated with different concentrations of fluconazole in the presence of ascorbic acid, H2O2, iron, iron plus H2O2, copper and copper plus ascorbic acid, followed by agarose gel electrophoresis. Fluconazole?DNA interactions were investigated by UV?Vis spectrophotometeric titration and in silico methods. Even in the presence of an oxidative agent or a reducing agent, at higher concentrations of fluconazole, double stranded DNA was not broken more than usually found in human plasma. Fluconazole concentrations ? 88 ?M could protect 46 ?M of DNA against hydroxyl radicals produced in the reaction between 1.5 mM of FeSO4 and 6 mM of H2O2 while drug concentrations ? 44 ?M could not provide the protection. In addition, the drug could not protect DNA against ROS originating from the reaction between copper and ascorbic acid. The binding constant of fluconazole?DNA in UV-Vis spectrophotometry analysis and docking analysis was estimated to be 1.087?103 and 6.22?105 M-1, respectively.

Publisher

National Library of Serbia

Subject

General Chemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3