Establishing the resistance of Myzus persicae (Sulzer) by molecular methods

Author:

Vucetic Andja1,Petrovic-Obradovic Olivera1ORCID,Margaritopoulos J.2,Skouras P.2

Affiliation:

1. Poljoprivredni fakultet, Beograd

2. Laboratory of Entomology and Agricultural Zoology, Department of Crop Production and Agricultural Environment, University of Thessaly, Volos, Greece

Abstract

In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investiga?tions, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance.

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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