Affiliation:
1. School of Economics and Management Fuzhou University, Fuzhou, China
2. Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Abstract
Identifying the accurate boundaries of a species is critical to have a better
perspective of any biological studies. Therefore, species delimitation is a
subject of extensive part of studies in the framework of biology. Erodium
species possess significant pharmacological and biological activities. The
whole plant was used as astringent and haemostatic in uterine and other
bleeding Therefore, due to the importance of these plant species, we
performed a molecular data analysis for this species. For this study, we
used 60 randomly collected plants from 5 species in five provinces.
Amplification of genomic DNA using 10 primers produced 52 bands, of which 50
were polymorphic (98.48%). The obtained high average PIC and MI values
revealed high capacity of ISSR primers to detect polymorphic loci among
Erodium species. The genetic similarities of five collections were estimated
from 0.77 to 0.91. According to Inter-Simple sequence repeats (ISSR) markers
analysis, E. cicutarium and E. malacoides had the lowest similarity and the
species of E. malacoides and E. oxyrrhynchum had the highest similarity. The
aims of present study are: 1) can ISSR markers identify Erodium species, and
2) to investigate the species inter-relationship? The present study revealed
that ISSR markers can identify the species.
Publisher
National Library of Serbia