Molecular characterization of oats (Avena sativa L.) diversity: Implications for dual-purpose breeding
Author:
Kaur Rajvir1ORCID, Kapoor Rahul1ORCID, Vikal Yogesh2ORCID, Amandeep A1ORCID
Affiliation:
1. Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, India 2. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Abstract
Oat (Avena sativa L.) is one of the world's healthiest and gluten-free grains
that are packed with essential vitamins, minerals, fiber, and antioxidants.
It is also one of the most important cereal fodder crops. The present study
was conducted from 2016 to 2018 and morphological and molecular diversity
was analyzed for dual-purpose oat based on ten fodder and eight grain traits
among 96 oats accessions including four wild accessions (A. vavilioviana,
Guiena oats, A. maroccana and A. sterilis) collected from various
eco-geographical regions of India. Thirty-one out of one hundred seventy
(18%) simple sequence repeats (SSR) markers detected polymorphism among the
96 oat accessions with average polymorphic information content (PIC) of
0.47. A total of 100 alleles were detected with an average of 3.2 alleles
per primer. The molecular diversity analysis grouped all the 96 germplasm
lines into two major clusters, ?A? and ?B?. The similarity coefficients
ranged from 0.37 to 1. The genotypic pairs viz; UPO 276: SKO 315 (46%); SKO
314: OL-125 (46%); SKO 314: OS 363 (49%); SKO 314: UPO 032 (49%) exhibited
least genetic similarity and these pairs can be potentially used as parents
to conduct various mapping studies and further contributing to the oat
breeding community. Moreover, 6 accessions (JHO-2001-1, JHO-99-2, OL 1635,
SKO 27, UPO 093 and OS329) had been identified which were superior to OL-10
(best check in the northwest India) for fodder as well as grain yield. This
study showed the opportunity of utilizing SSR markers with morphological
characteristics to breed for dual purpose oats.
Publisher
National Library of Serbia
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