Affiliation:
1. Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Nitra, Slovakia
Abstract
Broad spectrum antimicrobial agents are urgently needed to fight frequently
occurring multidrug-resistant pathogens. Myxobacteria have been regarded as
?microbe factories? for active secondary metabolites, and therefore, this
study was performed to isolate two bacteriolytic genera of myxobacteria,
Myxococcus sp. and Corallococcus sp., from 10 soil/sand samples using two
conventional methods followed by purification with the aim of determining the
antimicrobial activity of methanol extracts against 11 test microorganisms
(four Gram-positive, four Gram-negative, two yeasts and one fungus). Out of
thirty-nine directly observed strains, 23 were purified and analyzed for
antimicrobial activities. Based on the broth microdilution method, a total of
19 crude extracts showed antimicrobial activity. The range of inhibited wells
was more important in the case of anti-Gram-positive-bacterial activity in
comparison with the anti-Gram-negative-bacterial and antifungal activity. In
light of the established degree and range of antimicrobial activity, two of
the most active isolates (BNEM1 and SFEC2) were selected for further
characterization. Morphological parameters and a sequence similarity search
by BLAST revealed that they showed 99% sequence similarity to Myxococcus
xanthus ? BNEM1 (accession no. KX669224) and Corallococcus coralloides -
SFEC2 (accession no. KX669225). As these isolates had antimicrobial activity,
they could be considered for use in the development of antibiotics for
pharmaceutical use.
Publisher
National Library of Serbia
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
4 articles.
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