Activities of antioxidative enzymes during Chenopodium rubrum L. Ontogenesis in vitro

Abstract

For the short-day plant Chenopodium rubrum, a 14 h/10 h photoperiod is inductive for flowering, while continuous light (CL) is noninductive. Plants of one group were grown continuously under an inductive photoperiod, while in the other group flowering induction was delayed by 17 days of CL in order to separate on the time scale different developmental phases in plants of the same age. Regardless of the photoperiodic conditions the plants were exposed to, seed maturation occurred in 10 weeks. Activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were determined in different phases of development (vegetative growth, flowering, seed development, and maturation). The activities of antioxidative enzymes depended on both the phase of development and the photoperiod. In plants grown continuously under an inductive photoperiod, high CAT and POD activities were detected at the time of flowering and decreased during seed development and maturation. In plants in which flowering induction was delayed by 17 days of CL, the activities of POD and SOD were lowest in the vegetative phase of development and attained maximum values in the phase of seed maturation. In both groups of plants, the highest CAT activity was measured at the time of flowering.