Morphology and molecular characterization of Tylenchulus semipenetrans from citrus orchards in northern Iran
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Published:2023
Issue:3
Volume:68
Page:301-314
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ISSN:1450-8109
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Container-title:Journal of Agricultural Sciences, Belgrade
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language:en
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Short-container-title:J AGR SCI BELGRADE
Author:
Asadi Seyedeh1, Jamali Salar1, Ghadamyari Mohammad1, Motaghitalab Vahid2
Affiliation:
1. Faculty of Agricultural Sciences, Department of Plant Protection, University of Guilan, Rasht, Iran 2. Faculty of Engineering Sciences, Department of Textile Engineering, University of Guilan, Rasht, Iran
Abstract
Tylenchulus semipenetrans Cobb, 1913 is among some of the most economically
important plant-parasitic nematodes in the world. The nematode has been
identified as the causal agent of slow decline. Most studies estimate the
yield losses, due to T. semipenetrans, to range from 10% to 30%, depending
on the level of infection, aggressiveness of the nematode population, soil
characteristics, susceptibility of the rootstock, presence of other
pathogens and grove management practices. In order to identify the citrus
nematode in northern Iran, soil and root samples were collected from
infected trees. The second-stage juveniles were isolated from the soils by
the tray method. Eggs and females were extracted from roots by the
centrifugal-flotation technique. Morphological observations and molecular
evidence confirmed this population as T. semipenetrans. A phylogenetic tree
of T. semipenetrans populations was reconstructed based on 28S rRNA gene
sequences using RAxML. Morphologically, there is a slight difference between
the studied population and the reported populations of T. semipenetrans from
pomegranate and banana orchards in southern Iran. Phylogenetic analysis
showed the close relationship of the T. semipenetrans population from
northern Iran with other populations of this species. Based on molecular
analysis, Tylenchulus was identified as a monophyletic group. The
phylogenetic position and measurements of T. semipenetrans were provided.
Publisher
National Library of Serbia
Subject
Plant Science,Soil Science,Agronomy and Crop Science,Animal Science and Zoology
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