Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon-Reference-Cited by-同舟云学术

Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon

Published:2022-09-04 Issue: Volume:74 Page:
ISSN:0792-156X
Container-title:Israeli Journal of Aquaculture - Bamidgeh
language:en
Short-container-title:

Author:

Li Yun-Dong¹^ORCID,Zhou Fa-Lin¹,Yang Qi-Bin²,Jiang Song¹,Yang Li-Shi¹,Huang Jian-Hua¹,Chen Xu³,Mai Qianlin¹,Jiang Shi-Gui¹

Affiliation:

1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

2. Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China

3. Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, China

Abstract

A novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%~91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was highest expressed in the hepatopancreas and the lowest expressed in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon.

Publisher

AquacultureHub

Subject

Agronomy and Crop Science,Aquatic Science

Link

https://ija.scholasticahq.com/article/38101-molecular-cloning-expression-pattern-of-trypsin-gene-and-association-analysis-with-growth-traits-in-penaeus-monodon.pdf