Thermal stability of probiotic preparations in granulated combined feeds

Abstract

Purpose. To investigate the heat resistance of probiotic preparations before and after granulation of compound feed, to compare the obtained results. Methods. General scientific: hypothesis, experiment, analysis and synthesis. Specific: morphological, biochemical and statistical. To define the heat resistance of bacterial preparations, the colony-forming viability of bacterial cells was determined before and after granulation with exposure to maximum heat for one minute. The temperature of the granulated compound feed (before cooling) was set with a maximum thermometer. Results. It was established that increasing the temperature of the finished granules to 80-82°C significantly reduced the colony-forming ability of the cells of the bacterial preparation “BTU Probiotic”. Thus, at application of a 300 g dose of BTU probiotic per ton of compound feed, survival of bacterial colonies of the Bacillus subtilis strain decreased by 22.2%, at a 400 g dose of the preparation per ton of compound feed, survival of bacterial colonies decreased by 58.3%, at a 500 g dose – decreased by 53.3%. The maximum resistance to elevated temperatures and pressure was shown by the subtiform probiotic preparation with exposure of up to one minute. Thus, at application of 300 g doses of Subtiform per ton of compound feed, survival of bacterial colonies of another strain of Bacillus subtilis decreased by 16.0%, at 400 g doses of the preparation it decreased by 10%, at 500 g doses –decreased by 15.4%. Conclusions. The highest level of the Subtiform probiotic bacterial colonies survival was observed at application of a 400 g dose per ton of compound feed. Based on the obtained results, it can be stated that the heat resistance of the Subtiform probiotic preparation during granulation of compound feed was 38-48% higher compared to the “Probiotic BTU” preparation.