Profiling of Oral and Nasal Microbiome in Children with Cleft Palate

Author:

Zhang Min1,Wang Renke1,Liao Ying1,Buijs Mark Johannes2,Li Jiyao1

Affiliation:

1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

2. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam, and Free University Amsterdam, Amsterdam, the Netherlands.

Abstract

Objective To identify the oral and nasal microbial profile of cleft palate children and control children and to reveal interrelationships between the microbiome and the high prevalence of infectious diseases. Design Saliva and nasal samples of 10 cleft palate children and 10 age-matched control children were analyzed. Total microbial genomic DNA was isolated, polymerase chain reaction-denaturing gradient gel electrophoresis was applied to obtain fingerprints, and selected bands on fingerprints were sequenced. Results The results revealed a significantly lower saliva microbial diversity in cleft children and a different microbial component in both saliva and nares in children with cleft palate. A higher component similarity between the oral and nasal samples was found in the cleft group than in the control group. Lautropia species and Bacillus species were significantly less present among the saliva samples of cleft group. Dolosigranulum species and Bacillus species were significantly fewer in the nasal cavity of cleft group. Streptococcus species became much more predominant in the nasal cavity of the cleft group than in that of the control group. Conclusions A disturbed ecological ecosystem is found in oral and nasal microbiome of children with cleft palate as a consequence of the abnormal communication between the two cavities. Further studies are needed to explore the relationship between the disturbed microbiome and diseases.

Publisher

SAGE Publications

Subject

Otorhinolaryngology,Oral Surgery

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