Zoonotic and commensal bacteria from pigs with acquired antimicrobial resistance

Author:

Chechet O. N.,Gorbatyuk O. I.,Rublenko І. О.,Kuryata N. V.,Вuchkovska G. A.,Musiets I. V.,Shchur N. V.,Shalimova L. O.,Ordynska D. О.,Balanchuk L. V.,Togachynska L. V.

Abstract

The growing prevalence of foodborne zoonotic infections increases the risk of emergence of microorganisms with acquired resistance to antimicrobial drugs. Such microorganisms are capable of horizontal transmission of R-plasmids of antibiotic resistance to other types of bacteria. The results of our monitoring in 2021, in accordance with the State Strategy of Ukraine to curb the development of antimicrobial resistance and reduce the risks of the formation and spread of antimicrobial-resistant strains of microorganisms, showed high resistance of enterobacteria, enterococci and Campylobacter isolated from pigs to antibiotics of various groups. Among the isolated cultures of Esherichia coli, the ability of some strains to produce extended-spectrum β-lactamases (ESBL) was found. Testing of the experimental isolates for antibiotic resistance and screening for possible production of acquired resistance enzymes was carried out by the disc diffusion method with antibiotic disks of different groups. Out of 542 samples of appendix from pigs from farms in different regions of Ukraine, 138 isolates were isolated and identified. Among them: Esherichia coli – 68 strains; Enterococcus faecalis – 57; Enterococcus faecium – 9; Salmonella spp. – 2 and Campylobacter spp. – 2. Antibiotic resistance testing showed that 10 (7.3% of the isolates) of different bacterial species remained susceptibile to all antibiotics used. The remaining 128 test isolates (93.5% of the isolates) showed antibiotic resistance. The largest number of antibiotic-resistant commensal and zoonotic bacteria was detected in pig farms of Donetsk, Kyiv, Dnipro, Kirovohrad and Chernihiv regions. Escherichia coli and Enterococcus spp. were most often isolated from pigs; in smaller quantities Salmonella spp. and Campylobacter spp. All strains of Salmonella spp. faecium showed resistance to 1 to 3 antibiotics of different groups. Among the antibiotic-resistant E. faecalis, 1 strain with resistance to vancomycin was detected. Polyantibiotic resistance was inherent in E. coli strains. Among the polyantibiotic-resistant E. coli strains, 4 strains were found and confirmed to have acquired resistance due to the production of ESBL.

Publisher

Oles Honchar Dnipropetrovsk National University

Reference32 articles.

1. Akpinar, A., Saygili, D., & Yerlikaya, O. (2020). Production of set-type yoghurt using Enterococcus faecium and Enterococcus durans strains with probiotic potential as starter adjuncts. International Journal of Dairy Technology, 73, 637–803.

2. Bankovska, I. B., Voloshchuk, V. M., Podobied, L. I., & Smyslov, S. Y. (2016). Model optymizatsii vyrobnytstva yakisnoyi svynyny v suchasnykh umovakh tovarnoho svynarstva [A model for optimizing the production of high-quality pork in modern commercial pig farming conditions]. Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrayiny. Seriia: Tekhnolohiia vyrobnytstva i pererobky produktsii tvarynnytstva, 250, 114–124 (in Ukrainian).

3. Buckel, W. R., Veillette, J. J., Vento, T. J., & Stenehjem, E. (2018). Antimicrobial stewardship in community hospitals. The Medical Clinics of North America, 102(5), 913–928.

4. Campos, В. C., Fenner, I., Wiese, N., Lensing, C., Christner, M., Rohde H., Aepfelbacher, M., Fenner, T., & Hentschke, M. (2014). Prevalence and genotypes of extended spectrum beta-lactamases in Enterobacteriaceae isolated from human stool and chicken meat in Hamburg, Germany. Antimicrobial Resistance and Infection Control, 304(5–6), 678–684.

5. Champagne, J., Diarra, M. S., Rempel, H., Topp, E., Greer, C. W., Harel J., & Masson, L. (2011). Development of a DNA microarray for enterococcal species, virulence, and antibiotic resistance gene determinations among isolates from poultry. Applied and Environmental Microbiology, 77(8), 2625–2633.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3