Application of the thin-layer chromatography method for analysis of plant antioxidant activity-Reference-Cited by-同舟云学术

Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Published:2021-04-28 Issue:1 Volume:4 Page:26-30
ISSN:2618-7272
Container-title:Food systems
language:
Short-container-title:Food systems

Author:

Kupaeva N. V.¹^ORCID,Vasilevskaya E. R.¹^ORCID,Fedulova L. V.¹^ORCID,Kotenkova E. A.¹^ORCID

Affiliation:

1. V.M. Gorbatov Federal Research Centre for Food Systems, RAS

Abstract

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

Publisher

The Gorbatov's All-Russian Meat Research Institute

Reference19 articles.

1. Mirovich, V.M., Privalova E. G. (2018). Biologically active substances of plants (polysaccharides, essential oils, phenologlycosides, coumarins, flavonoids): Textbook. Irkutsk State Medical University. — 70 p. (In Russian)

2. Menshchikova, E.B., Lankin, V.Z., Kandalintseva N. V. (2012) Phenolic antioxidants in biology and medicine. Structures, properties, mechanisms of action: LAP Lambert Academic Publishing. — 496 p. ISBN 9783846594612. (In Russian)

3. Gorovoy, P.O., Balyshev, M.E. (2017). Possibilities and prospect applications of medicinal plants in Russian Far East Pacific Medical Journal, 3(69), 5-14. https://doi.org/10.17238/PmJ1609-1175.2017.3.5-14 (In Russian)

4. Soyer, A., Ozalp, B., Dalmi§, U., Bilgin, V. (2010). Effects of freezing temperature and duration of frozen storage on lipid and protein oxidation in chicken meat. Food Chemistry, 120(4), 1025-1030. https://doi.org/10.1016/j.foodchem.2009.11.042

5. Gurinovich, G.V., Patrakova, I.S., Kudryashov, L.S. (2018). Study of the effect of curing mixture compositions on oxidation of lipids in meat systems. S Food Processing: Techniques and Technology, 48(1), 31-40. https://doi.org/10.21603/2074-9414-2018-1-31-40 (In Russian)