Optimization of UV Spectrophotometric Method for Estimation of Darunavir in Bulk Drug and Tablet Formulations

Abstract

A simple and cost effective spectrophotometric method has been developed and subsequent validation of Darunavir in its bulk and formulation with good accuracy and precision. An absorption maximum was determined at 254 nm. Beer’s law was obeyed in the concentration range of 10-35µg/ml with correlation coefficient of 0.998. Darunavir is a new protease inhibitor, is a retroviral drug used in treatment of (HIV) Type-1 infection. The % assay for Darunavir ranged from 98.4-102.1% in tablet dosage form. Hence the developed method can be used for routine analysis of Darunavir in pharmaceutical formulations