Standardization of Suitable Substrate and Buffer Solutions for Optimum in-vivo Nitrate Reductase Activity in the Leaves of Sesbania rostrata Bremek. and Oberm.-Reference-Cited by-同舟云学术

Standardization of Suitable Substrate and Buffer Solutions for Optimum in-vivo Nitrate Reductase Activity in the Leaves of Sesbania rostrata Bremek. and Oberm.

Published:2016-01-06 Issue:2 Volume:39 Page:121-124
ISSN:0971-9431
Container-title:Indian Journal of Forestry
language:en
Short-container-title:Indian J. Forest.

Author:

Chaukiyal S.P.¹,Vandana ¹,Uniyal Poornima²

Affiliation:

1. Forest Research Institute

2. Dayanand Brijendra Swarup Post Graduate College

Abstract

Sesbania rostrata Bremek. and Oberm. is a multipurpose and exceptionally fast growing nitrogen fixing plant. In this study a protocol for the standardization of in-vivo nitrate reductase (NR) assay with respect to substrate concentrations along with buffer pH and concentrations was carried out in the leaves of S. rostrata. Different substrate (KNO3) concentration ( i.e. 0.025M, 0.05M, 0.10M, 0.15M, 0.20M ) were tried against phosphate buffer (KH2PO4) solutions with different pH (6.5, 7.0, 7.5, 7.6, 7.7, 7.8 ). Maximum leaf in-vivo nitrate reductase activity was observed in 0.10 M phosphate buffer (KH2PO4) concentration of pH 7.5 with 0.15M substrate (KNO3) concentration.

Publisher

Bishen Singh Mahendra Pal Singh

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