Affiliation:
1. Forest Research Institute
2. National Institute of Technology
Abstract
Coleus barbatus (Patharchur) - an important medicinal species was multiplied through in vitro techniques in response to various growth regulatory hormones. Sterilization of explants was carried out with 0.1% Mercuric chloride for 10 min. Inoculation of explants on MS (Murashige and Skoog, 1962) medium supplemented with lower concentration of auxin NAA (0.25mg/L) and higher concentration of cytokinin BAP (1mg/L) proved quite effective. The maximum rooting % (100%) and maximum number of roots (8) were found in plants cultured on ½ strength MS media supplemented with NAA (1mg/L) while the length of roots was maximum with IBA (1mg/L). The plantlets were hardened, acclimatized and successfully transferred to the field conditions which showed 96% survival.
Publisher
Bishen Singh Mahendra Pal Singh
Reference18 articles.
1. Abraham, Z., Srivastava, A.K. and Bagchi, G.D. (1988). Cytoplasmic vesicles containing secondary metabolites in the root of Coleus forskohlii (Willd.) . Briq. Current Science, 57: 1339-1377
2. Ammon, H.P.T. and M?ller, M. (1985). Forskolin: from Ayurvedic remedy to a modern agent. Planta Medica. 46: 473-477.
3. Bhattacharya, R. and Bhattacharya S. (2001). In vitro multiplication of Coleus forskohlii Briq. An approach towards shortening the protocol. In vitro cellular and Devleopmental. Biology – Plant, 37: 572-575.
4. Chowdhary, A.R. and Sharma, M.L. (1998). GC-MS investigations on the essential oil from Coleus forskohlii Briq. Indian Perfumer, 42: 15-16
5. Datta, K., Datta, K. and Pramanik, T. (1983). In vitro clonal multiplication of mature trees of Dalbergia sissoo Roxb. Plant Cell, Tissue and Organ Culture, 2: 15-20.