Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress

Author:

Alsamman Alsamman M.1,Bousba Ratiba2,Baum Michael3,Hamwieh Aladdin4,Fouad Nourhan4

Affiliation:

1. MGGM laboratory, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Egypt

2. Genetics, Biochemistry, and Plant Biotechnology Laboratory, Department of Biology and Plant Ecology, Mentouri Brothers University, Algeria

3. International Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 6299, Rabat, Morocco

4. International Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 2416, Cairo, Egypt

Abstract

Heat and drought are among the leading environmental stresses which have a major impact on plant development. In our research, identification and characterization of differentially expressed genes (DEGs) regulating the response of wheat to drought, heat and combined stress was carried out. We analyzed data from the Gene Expression Omnibus database (GEO) microarrays containing 24 samples of wheat, which were categorized by different treatments (control: ctrl, drought: D, heat: H, and mixed: HD). Significant DEGs were examined for gene annotation, gene ontology, co-expression, protein-protein interaction (PPI) and their heterogeneity and consistency through drought, heat and combined stress was also studied. Genes such as gyrB, C6orf132 homolog, PYR1 were highly associated with wheat response to drought with P-value (-log10) of 9.3, 7.3, 6.4, and logFC of -3.9, 2.0, 1.6, respectively. DEGs associated with drought tolerance were highly related to the protein domains of lipid-transfer (LTP). Wheat response to heat stress was strongly associated with genes such as RuBisCO activase B, small heat shock, LTP3, YLS3, At2g33490, PETH with p-values (-log10) ranging from 9.3 to 12.3. In addition, a relatively high number of protein interactions involved the SDH, PEPCK, and G6PD genes under heat stress.

Publisher

International Library of Science

Subject

General Engineering

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