New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR

Author:

Gallup Jack M.,Kawashima Kenji,Lucero Ginger,Ackermann Mark R.

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Reference24 articles.

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3. Use of glycogen or tRNA for reducing non-specific adherence of templates to Plastic. Applied Biosystems Incorporated 5700 User Manual 2000. Section 5, p.14.

4. Lee J, Duncan A, Warrick S. Techniques for Optimizing RT-PCR Reactions. PharmaGenomics 2003: pp. 54–55.

5. Dunphy J, Horvath A, Barcham G, Balic A, Bischof R, Meeusen E. Isolation, characterisation and expression of mRNAs encoding the ovine CC chemokines, monocyte chemoattractant protein (MCP)-1α and -2. Vet Immunol Immunopathol 2001; 82(3-4):153–164.

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