Determination of the Pharmacokinetics of Florfenikol in Turbot (Psetta maxima) Fish Grown in Black Sea Water

Abstract

In this study, it was aimed to determine the kinetics of florfenicol (FF) given intramuscularly and gavage in a single dose (10 mg/kg) in Black Sea Turbot (Psetta maxima), whose growing possibilities were investigated in Turkey in recent years. The study was carried out in black sea water, in closed system pools within the Trabzon Fisheries Central Research Institute. During the experiment, the Black Sea water temperature was 11±1 celsius, the salinity rate was 0.18%, the daily water change was carried out 15 times with a water flow rate of 1 liter/ minute. The amount of oxygen did not fall below 10.02 mg/ml. Experiments were performed on 65 turbot fish (42±2 g), which underwent bacterial and parasitic examination before the trial, including validation (5 specimens) and drug trials (60 specimens). Turbot fish were divided into two groups as 10 mg/kg body weight (c.a) FF administered intramuscularly and gavage. For the validation study, blood taken from turbot fish that were not given medication was used. Determination of plasma FF levels was performed on a high pressure liquid chromatography device (HPLC). Pharmacokinetic analyzes were performed using the win-nonlin pharmacokinetic program according to the non-compartmental model. The results of the validation studies were determined as 96.19, 85.81±0.026, 0.0039 and 0.012 based on Relative Standard Deviation (RSD) %, mean recovery %, LOD and LOQ in ppm, respectively. Tmax (hour), Cmax (mg L-1), Tlast (hour), Clast (mg L-1), AUClast (hour mg L-1), Vz_F_obs (L kg-1) of intramuscular and gavage FF administration groups, Cl_F_obs (L kg h-1) values are 1 and 6, respectively; 6.60 and 5.17; 48 and 48, 2.02 and 1.48, 145.39 and 101.76, 2.54 and 3.64, 0.033 and 0.045. The results of the study showed that the therapeutic dose range of FF can be extended up to 48 hours at 11±1°C in turbot fish, according to the MIC literature information for agents in fish.