1. the samples were collected and gently washed with cold PBS. Next, the samples were crushed and immersed in 1% sodium dodecyl sulfate (SDS) solution for 2 h to extract absorbed proteins under shaking. The amounts of proteins absorbed on different scaffolds were quantified by a BCA Protein Assay Kit (Thermofisher, USA);addition, after subcutaneous implantation in rats for 7 days, the expression of CD105 (1:500 dilution, ab252345, Abcam), (1:500 dilution, ab192256, Abcam) and BMP2 (1:100 dilution, ab284387, Abcam) was also evaluated using immunofluorescence stainingimmunofluorescent staining. Immunofluorescent staining of CD68 (1:100 dilution, ab283654, Abcam), CD206 (1:100 dilution, ab300621, Abcam) and iNOS (1:50 dilution
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